Solid phase chemistry to covalently and reversibly capture thiolated RNA

An Introduction to Solid - Phase Palladium Chemistry

[Selective metalation and solid phase organometallic chemistry].

Recently, solid-phase synthesis has been recognized as an important methodology for combinatorial chemistry or automated synthesis directed at drug discovery. Various synthetic methodologies have been applied to solid-phase synthesis, although it appears that organometallic chemistry in this area is still being explored. In solution-phase chemistry, organometallic reagents have been utilized fo...

Redox-responsive, reversibly-crosslinked thiolated cationic helical polypeptides for efficient siRNA encapsulation and delivery.

Cationic helical polypeptides, although highly efficient for inducing membrane penetration, cannot stably condense siRNA molecules via electrostatic interactions, which greatly limit the gene knockdown efficiency. By developing and crosslinking the thiolated polypeptide via formation of disulfide bonds post formation of the polypeptide/siRNA complexes, we were able to obtain stable complexes wi...

Binding of ribosomal proteins to RNA covalently coupled to agarose.

An affinity chromatographic procedure, using ribosomal or tRNA covalently coupled to agarose through an adipic acid dihydrazide spacer, has been developed to isolate and identify the Escherichia coli ribosomal proteins which specifically bind to these RNA molecules. Transfer RNA, 5-S, 16-S and 23-S ribosomal RNA were readily immobilized on agarose-dihydrazide: the maximum amount coupled decreas...

Perspectives on moving ionic liquid chemistry into the solid phase.

Ionic liquid (IL) chemistry has evolved over the past century, such that these organic salts have impacted virtually every area of science and engineering. In the area of chemistry, initial applications of these salts were primarily the domain of chemists or chemical engineers who desired to manipulate the properties of IL solvents for a variety of applications including tuning various chemical...