SOFI-based 3D superresolution sectioning with a widefield microscope
نویسندگان
چکیده
منابع مشابه
Multiplane 3D superresolution optical fluctuation imaging
By switching fluorophores on and off in either a deterministic [1, 2] or a stochastic [3–7] manner, superresolution microscopy has enabled the imaging of biological structures at resolutions well beyond the diffraction limit. Superresolution optical fluctuation imaging (SOFI) [7, 8] provides an elegant way of overcoming the diffraction limit in all three spatial dimensions by computing higher-o...
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Superresolution Optical Fluctuation Imaging (SOFI) as initially demonstrated allows for a resolution enhancement in imaging by a factor of square-root of two. Here, we demonstrate how to increase the resolution of SOFI images by re-weighting the Optical Transfer Function (OTF). Furthermore, we demonstrate how cross-cumulants can be exploited to obtain a fair approximation of the underlying Poin...
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Most current superresolution (SR) microscope techniques surpass the diffraction limit at the expense of temporal resolution, compromising their applications to live-cell imaging. Here we describe a new SR fluorescence microscope based on confocal microscope optics, which we name the spinning disk superresolution microscope (SDSRM). Theoretically, the SDSRM is equivalent to a structured illumina...
متن کاملImproved sectioning in a slit scanning confocal microscope.
We describe a simple implementation of a slit scanning confocal microscope to obtain an axial resolution better than that of a point-scanning confocal microscope. Under slit illumination, images of a fluorescent object are captured using an array detector instead of a line detector so that out-of-focus light is recorded and then subtracted from the adjacent images. Axial resolution after backgr...
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ژورنال
عنوان ژورنال: Optical Nanoscopy
سال: 2012
ISSN: 2192-2853
DOI: 10.1186/2192-2853-1-2