Site-directed ligand discovery

Site-directed ligand discovery.

We report a strategy (called "tethering") to discover low molecular weight ligands ( approximately 250 Da) that bind weakly to targeted sites on proteins through an intermediary disulfide tether. A native or engineered cysteine in a protein is allowed to react reversibly with a small library of disulfide-containing molecules ( approximately 1,200 compounds) at concentrations typically used in d...

AMPA receptor ligand-binding domain: Site-directed mutagenesis study of ligand-receptor interactions

Academic Dissertation To be presented for public criticism, with the permission of the Faculty of Science of the (1998) Disulfide bonding and cysteine accessibility in the α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor subunit GluRD. Implications for redox modulation of glutamate receptors. receptors and bacterial periplasmic amino acid-binding proteins share the ionic mechanism ...

Epipaleolithic Site Discovery in Southeastern of Iran, Rayen

In the spring of 2012 an archaeological survey was conducted within the Rayen region in order to explore all the archaeological periods in this region and document all archaeological settlement patterns of this region. In general, 52 archaeological sites were discovered and documented which included all prehistoric and historic sites. Among these sites, three of them seem to belong to the Paleo...

Site-Directed Mutagenesis Study

Structural elements of the rat 1.t-opioid receptor important in ligand receptor binding and selectivity were examined using a site-directed mutagenesis approach. Five single amino acid mutations were made, three that altered conserved residues in the ~ 8, and K receptors (Asn 15°t Ala, His297 to Ala, and Tyr326 to Phe) and wo de igned to test for ~i/8 selectivity (lie198 to Val and Va1202 to li...

Site-Directed Covalent Immobilization