SISCAPA Peptide Enrichment on Magnetic Beads Using an In-line Bead Trap Device
نویسندگان
چکیده
منابع مشابه
SISCAPA peptide enrichment on magnetic beads using an in-line bead trap device.
A SISCAPA (stable isotope standards and capture by anti-peptide antibodies) method for specific antibody-based capture of individual tryptic peptides from a digest of whole human plasma was developed using a simplified magnetic bead protocol and a novel rotary magnetic bead trap device. Following off-line equilibrium binding of peptides by antibodies and subsequent capture of the antibodies on ...
متن کاملElectrochemical detection of insulating beads at subattomolar concentration via magnetic enrichment in a microfluidic device.
We report electrochemical detection of collisions between individual magnetic microbeads, present at subattomolar concentrations, and electrode surfaces. This limit of detection is 4 orders of magnitude lower than has been reported previously, and it is enabled by using a magnetic field to preconcentrate the microbeads prior to detection in a microfluidic electrochemical cell. Importantly, the ...
متن کاملComparison of non-magnetic and magnetic beads in bead-based assays.
Multiplex bead-based flow cytometry is an attractive way for simultaneous, rapid and cost-effective analysis of multiple analytes in a single sample. Previously, we developed various bead-based assays using non-magnetic beads coated with Staphylococcus aureus and Streptococcus pneumoniae antigens for the detection of antibodies. Here, we compared the performance of the assay using non-magnetic ...
متن کاملHigh-Throughput SISCAPA-Based Peptide Quantitation Using an Agilent RapidFire High-Throughput Mass Spectrometry System
A high-throughput SISCAPA workfl ow has been developed that uses RapidFire/MS technology to enable sensitive quantitation of peptides from plasma digests with a sample cycle time of approximately 10 seconds. The optimized system includes capture of target proteotypic peptides using rigorously selected, high affi nity antipeptide monoclonal antibodies and reduction of background peptides using a...
متن کاملMierofluidic mixer using magnetic beads
In this paper, we present a novel, simple and low-cost micromixer that is based on the manipulation of self-assembled structures by an alternating magnetic field of magnetic microbeads over the section of a microfluidic channel. The principle is demonstrated using a microfluidic chip of polymethylmetacrylate (PMMA) that contains a 200 pm wide Y-shaped microchannel and is integrated with soft fe...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2009
ISSN: 1535-9476
DOI: 10.1074/mcp.m800446-mcp200