Single-target regulators form a minor group of transcription factors in Escherichia coli K-12

Escherichia coli K-12 F-prime factors, old and new.

Transduction in Escherichia Coli K-12.

SYSTEM of genetic transduction has been discovered in the sexually fertile A K-12 strain of Escherichia coli. This transduction is mediated by lambda, a temperate phage for which K-12 is normally lysogenic. The distinctive features of the lambda-K-12 system include the following: (1) The transductions are limited to a cluster of genes for galactose fermentation. The GaZ loci are closely linked ...

The repertoire of DNA-binding transcriptional regulators in Escherichia coli K-12.

Using a combination of several approaches we estimated and characterized a total of 314 regulatory DNA-binding proteins in Escherichia coli, which might represent its minimal set of transcription factors. The collection is comprised of 35% activators, 43% repressors and 22% dual regulators. Within many regulatory protein families, the members are homogeneous in their regulatory roles, physiolog...

Hfr Strains of Escherichia coli K-12

Since the first reports by Cavalli-Sforza (9) and Hayes (24) of strains of Escherichia coli K-12 which are conjugational donors of chromosomal markers at high frequency (Hfr strains), many other Hfr strains have been isolated and our understanding of the mechanisms involved in Hfr formation has increased considerably. All Hfr strains arise from the integration of a conjugative plasmid into the ...

Phosphoglucomutase mutants of Escherichia coli K-12.

Bacteria with strongly depressed phosphoglucomutase (EC 2.7.5.1) activity are found among the mutants of Escherichia coli which, when grown on maltose, accumulate sufficient amylose to be detectable by iodine staining. These pgm mutants grow poorly on galactose but also accumulate amylose on this carbon source. Growth on lactose does not produce high amylose but, instead, results in the inducti...