Single Spheroid Metabolomics: Optimizing Sample Preparation of Three-Dimensional Multicellular Tumor Spheroids
نویسندگان
چکیده
منابع مشابه
differential thermo-resistance of multicellular tumor spheroids
many cell lines, when cultured under proper conditions, can form three dimensionalstructures called multicellular spheroids. these spheroids resemble in vivo tumor models in severalaspects. therefore, studying growth characteristics and behavior of spheroids is beneficial inunderstanding the behavior of tumors under various experimental conditions. in this work, we havestudied the growth proper...
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Invasion of surrounding normal tissues is generally considered to be a key hallmark of malignant (as opposed to benign) tumors. For some cancers in particular (e.g., brain tumors such as glioblastoma multiforme and squamous cell carcinoma of the head and neck - SCCHN) it is a cause of severe morbidity and can be life-threatening even in the absence of distant metastases. In addition, cancers wh...
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The in vitro growth of small (0.05 to 3 mm diameter) avascular multicellular tumor spheroids from six rodent and two human tumor lines has been analyzed. Surprisingly, the radial increase of multicellular tumor spheroids is linear with time after a brief initial period of geometric growth. These multicellular tumor spheroids are shown to have a constant thickness of proliferative outer crust an...
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The precise role of the microenvironment on tumor growth is poorly understood. Whereas the tumor is in constant competition with the surrounding tissue, little is known about the mechanics of this interaction. Using a novel experimental procedure, we study quantitatively the effect of an applied mechanical stress on the long-term growth of a spheroid cell aggregate. We observe that a stress of ...
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Most organisms grow according to simple laws, which in principle can be derived from energy conservation and scaling arguments, critically dependent on the relation between the metabolic rate B of energy flow and the organism mass m. Although this relation is generally recognized to be of the form B(m) = m, the specific value of the exponent p is the object of an ongoing debate, with many mecha...
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ژورنال
عنوان ژورنال: Metabolites
سال: 2019
ISSN: 2218-1989
DOI: 10.3390/metabo9120304