Single-molecule RNA Fluorescence in situ Hybridization (smFISH) in Caenorhabditis elegans

Single molecule fluorescent in situ hybridization ( smFISH ) of C . elegans worms and embryos *

To whom correspondence should be addressed. E-mail: [email protected]. Single molecule fluorescent in situ hybridization (smFISH) of C. elegans worms and embryos Ni Ji and Alexander van Oudenaarden Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139 USA Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139...

Single molecule fluorescent in situ hybridization (smFISH) of C. elegans worms and embryos.

Single-molecule fluorescence in situ hybridization: Quantitative imaging of single RNA molecules

In situ detection of RNAs is becoming increasingly important for analysis of gene expression within and between intact cells in tissues. International genomics efforts are now cataloging patterns of RNA transcription that play roles in cell function, differentiation, and disease formation, and they are demonstrating the importance of coding and noncoding RNA transcripts in these processes. Howe...

Visualization of lncRNA by single-molecule fluorescence in situ hybridization.

Single-molecule RNA fluorescence in situ hybridization is a technique that holds great potential for the study of long noncoding RNA. It enables quantification and spatial resolution of single RNA molecules within cells via hybridization of multiple, labeled nucleic acid probes to a target RNA. It has recently become apparent that single-molecule RNA FISH probes targeting noncoding RNA are more...

Small RNA in situ hybridization in Caenorhabditis elegans, combined with RNA-seq, identifies germline-enriched microRNAs☆

Over four hundred different microRNAs (miRNAs) have been identified in the genome of the model organism the nematode Caenorhabditis elegans. As the germline is dedicated to the preservation of each species, and almost half of all the cells in an adult nematode are germline, it is likely that regulatory miRNAs are important for germline development and maintenance. In C. elegans the miR35 family...