Single-day FISH procedure for paraffin-embedded tissue sections using a microwave oven

Single-day FISH procedure for paraffin-embedded tissue sections using a microwave oven.

The fluorescence in situ hybridization (FISH) technique is a sensitive method for detecting gene amplification in tumor cells. However, FISH on formalin-fixed and paraffin-embedded tissue sections is time-consuming and labor-intensive (1). Tissue processing and cross-linking fixatives such as formalin may prevent the probe from accessing its target sequence, prolonging the FISH processing time ...

Efficient nuclear FISH on paraffin-embedded tissue sections using microwave pretreatment.

Multiparameter immunofluorescence on paraffin-embedded tissue sections.

Immunohistochemical techniques have gained increasing importance in diagnostics and research. While formalin-fixed, paraffin-embedded human tissue retains excellent morphology, the detection of antigens by immunofluorescence in its sections and especially the demonstration of multiple simultaneous antibodies have limitations. Double immunofluorescence labeling of routinely processed paraffin se...

Non-disruptive PNA-FISH protocol for formalin-fixed and paraffin-embedded tissue sections.

manganese in RT-PCR eliminates PCR artifacts resulting from DNase I digestion. BioTechniques 22:1128-1132. 3.Bolotin, A., S. Mauger, K. Malarme, S. Ehrlich, and A. Sorokin. 1999. Low-redundancy sequencing of the entire Lactococcus lactis IL1403 genome. Antonie Van Leeuwenhoek 76:27-76. 4.Buchman, G., D. Schuster, and A. Rashtchian. 1993. Selective RNA amplification: a novel method using dUMP-co...

Combined FISH and immunolabeling on paraffin-embedded tissue sections for the study of microchimerism.

Fluorescence in situ hybridization (FISH) has proven to be a very useful technique for the study of chimerism and microchimerism (2,4). Based on the detection of sex chromosomes, this technique allows for the identification and localization of donor and recipient cells in cases of sex-mismatched transplantation or cells from mothers and their male fetuses in studies of fetomaternal cell traffic...