Single Cell Trapping by Superhydrophobic/Superhydrophilic Microarrays

Single cell trapping is demonstrated on superhydrophobic–superhydrophilic patterned microarrays. Superhydrophilic spots that are twice the size of cells to be trapped found optimal for obtaining single trapping. The based exclusivity instead heavy dilution suspension and relying Poisson statistics. superhydrophobic areas array very resistant toward unwanted adhesion thus cleaning steps not needed after deposition. Based these properties, 20 μm superhydrophilic utilized two types immune cells, primary peripheral blood mononuclear (PBMCs) THP-1 cells. from a 10 μl droplet dragged across surface with different velocities. efficiencies in range 10–30% shown type, spot seeding velocity concentration.