SINEs as Potential Expression Cassettes: Impact of Deletions and Insertions on Polyadenylation and Lifetime of B2 and Ves SINE Transcripts Generated by RNA Polymerase III

Short Interspersed Elements (SINEs) are common in the genomes of most multicellular organisms. They transcribed by RNA polymerase III from an internal promoter comprising boxes A and B. As transcripts certain SINEs mammalian can be polyadenylated, such should contain AATAAA sequence as well those called β- τ-signals. One goals this work was to evaluate how autonomous independent other SINE parts Extended regions outside τ-signals were deleted B2 Ves derived constructs used transfect HeLa cells order relative levels their polyadenylation efficiency. If affected B, 5′-flanking region U6 gene with external inserted upstream. Such substitution completely restored its transcription. Almost all tested deletions/substitutions did not reduce capacity transcripts, indicating a weak dependence function on neighboring sequences. similar analysis containing 55-bp foreign between showed equal efficiency compared without insertion. The acquired poly(A)-tails significantly increased lifetime thus cellular level transcripts. data obtained highlight potential cassettes for expression relatively short sequences various applications.