Simultaneous tracking of fly movement and gene expression using GFP

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Simultaneous tracking of fly movement and gene expression using GFP

BACKGROUND Green Fluorescent Protein (GFP) is used extensively as a reporter for transgene expression in Drosophila and other organisms. However, GFP has not generally been used as a reporter for circadian patterns of gene expression, and it has not previously been possible to correlate patterns of reporter expression with 3D movement and behavior of transgenic animals. RESULTS We present a v...

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BACKGROUND In vivo imaging and quantification of fluorescent reporter molecules is increasingly useful in biomedical research. For example, tracking animal movement in 3D with simultaneous quantification of fluorescent transgenic reporters allows for correlations between behavior, aging and gene expression. However implementation has been hindered in the past by the complexity of operating the ...

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Design and Construction of ctxB-gfp-stxB Gene Cassette and Investigation of Its Expression in E. coli Bl21 (DE3)

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ژورنال

عنوان ژورنال: BMC Biotechnology

سال: 2008

ISSN: 1472-6750

DOI: 10.1186/1472-6750-8-93