Simultaneous Feulgen densitometry and autoradiographic grain counting with the Quantimet 720D image-analysis system. III. Improvements in Feulgen densitometry.
نویسندگان
چکیده
منابع مشابه
Analysis of nuclear DNA content in Capsicum (Solanaceae) by flow cytometry and Feulgen densitometry.
Flow cytometric measurements of nuclear DNA content were performed using ethidium bromide as the DNA stain (internal standard, Hordeum vulgare 'Ditta', 1C = 5.063 pg) in 25 samples belonging to nine diploid species and four varieties of Capsicum: C. chacoense, C. parvifolium, C. frutescens, C. chinense, C. annuum var. annuum, C. baccatum var. baccatum, C. baccatum var. pendulum, C. baccatum var...
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Despite of a huge amount of literature on the Feulgen reaction for DNA relatively few in vestigations deal with the special needs of plant scientists. As a consequence various mod ifications of the method are practised in different laboratories, which may in part be re sponsible for contradictory results. In the present work tests are conducted to find out, which steps of the procedure must ...
متن کاملVideo and scanning microdensitometer-based imaging systems in autoradiographic densitometry.
The characteristics of computer-assisted imaging devices (CIDs) incorporating charge-coupled device cameras, vidicon cameras and scanning microdensitometers (SMDs) are compared. Advantages of vidicons include their low cost, the option of real-time imaging, continuously variable optical magnification and the wide choice of available vidicon elements. Disadvantages include densitometric non-line...
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This study describes an image processing method for the assessment of the mitotic count in Feulgen-stained breast cancer sections. The segmentation procedure was optimized to eliminate 95-98% of the nonmitoses, whereas 11% of the mitoses did not survive the segmentation procedure. Contour features and optical density measurements of the remaining objects were computed to allow for classificatio...
متن کاملFlow Cytometric Analysis of Megakaryocyte Ploidy . Comparison With Feulgen
Flow cytometry (FCM) was adapted to measure ploidy values in megakaryocytes isolated from normal guinea pigs and African green monkeys. The FCM approach was validated by microscopic identification of electronically sorted SN and higher ploidy cells and by comparisons with Feulgen microdensitometry on the same samples. Sorted 8N populations contained only megakaryocytes and were completely free ...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1983
ISSN: 0022-1554,1551-5044
DOI: 10.1177/31.10.6886380