Simultaneous DNA and RNA isolation from brain punches for epigenetics

Method for simultaneous RNA and DNA isolation from biopsy material, culture cells, plants and bacteria.

The analysis of RNA and DNA in clinical biopsy material for diagnostic and research purposes has become more and more important. Currently available methods and kits are used to focus on the extraction of only one kind of nucleic acid, but since the amount of biopsy material is often limited, a method for the simultaneous isolation of both kinds of nucleic acids from one sample is desirable. In...

Simple and safe method for simultaneous isolation of microbial RNA and DNA from problematic populations.

We describe a novel, rapid, and safe method for extracting RNA and DNA from refractory microbes, which avoids the use of phenol or chloroform. It has been used successfully to isolate high-quality nucleic acids from pure cultures and environmental populations known to resist widely used extraction protocols.

SIDR: simultaneous isolation and parallel sequencing of genomic DNA and total RNA from single cells.

Simultaneous sequencing of the genome and transcriptome at the single-cell level is a powerful tool for characterizing genomic and transcriptomic variation and revealing correlative relationships. However, it remains technically challenging to analyze both the genome and transcriptome in the same cell. Here, we report a novel method for simultaneous isolation of genomic DNA and total RNA (SIDR)...

Simultaneous recovery of RNA and DNA from soils and sediments.

Recovery of mRNA from environmental samples for measurement of in situ metabolic activities is a significant challenge. A robust, simple, rapid, and effective method was developed for simultaneous recovery of both RNA and DNA from soils of diverse composition by adapting our previous grinding-based cell lysis method (Zhou et al., Appl. Environ. Microbiol. 62:316-322, 1996) for DNA extraction. O...

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*