Simplified template preparation and improved direct sequencing using Taq polymerase.
نویسندگان
چکیده
منابع مشابه
Simplified template preparation and improved direct sequencing using Taq polymerase.
A streamlined version of direct dideoxy sequencing is presented that includes template preparation as well as sequencing protocols. The method is used routinely to sequence double-stranded PCR products after minimal purification with one of the primers used in amplification. Either 35S or 32P labeling can be used with equally good results.
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ژورنال
عنوان ژورنال: Genome Research
سال: 1992
ISSN: 1088-9051
DOI: 10.1101/gr.1.4.255