Simplified, rapid DNA extraction protocol for STR typing from bones
نویسندگان
چکیده
منابع مشابه
Rapid DNA Extraction Protocol from Stool, Suitable for Molecular Genetic Diagnosis of Colon Cancer
Colorectal cancer (CRC) is one of the most common forms of cancers in the world and is curable if diagnosed at the early stage. Analysis of DNA extracted from stool specimens is a recent advantage to cancer diagnostics. Many protocols have been recommended for DNA extraction from stool, and almost allof them are difficult and time consuming, dealing with high amount of toxic materials like phen...
متن کاملStr Typing from Bloodstained Fabric without Dna Extraction
Whole blood from various individuals was spotted on 100% white cotton fabric. Once the bloodstains were dry, approximately 1 mm or less of the bloodstained thread was cut from each sample and placed in an autoclaved reaction tube containing either TE buffer or sterile distilled water. The tubes were then subjected to a simple preincubation period as described by the author previously (1). After...
متن کاملrapid dna extraction protocol from stool, suitable for molecular genetic diagnosis of colon cancer
colorectal cancer (crc) is one of the most common forms of cancers in the world and is curable if diagnosed at the early stage. analysis of dna extracted from stool specimens is a recent advantage to cancer diagnostics. many protocols have been recommended for dna extraction from stool, and almost allof them are difficult and time consuming, dealing with high amount of toxic materials like phen...
متن کاملA simplified universal genomic DNA extraction protocol suitable for PCR.
Conventional genomic DNA extraction protocols need expensive and hazardous reagents for decontamination of phenolic compounds from the extracts and are only suited for certain types of tissue. We developed a simple, time-saving and cost-efficient method for genomic DNA extraction from various types of organisms, using relatively innocuous reagents. The protocol employs a single purification ste...
متن کاملStr-typing of Nuclear Dna from Human Fecal Matter
Human fecal matter is routinely encountered in forensic casework—identifying the source of this material could link a suspect or victim to a crime scene. Previous methods used to identify the source of the fecal matter have primarily involved microscopic comparisons. While these methods can be useful, two sources of fecal matter are required, and typically the analysis cannot individualize the ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Forensic Science International: Genetics Supplement Series
سال: 2019
ISSN: 1875-1768
DOI: 10.1016/j.fsigss.2019.10.108