Simple reagent sparing approach to immunocytochemical staining of haematological and other samples with APAAP technique.
نویسندگان
چکیده
منابع مشابه
Automation of APAAP immunocytochemical technique.
A tissue processing instrument (the Histokinette) was modified by the addition of an electronic timing device which allows an immunocytochemical staining technique (the APAAP method) to be performed as a semiautomated procedure. After incubation with primary monoclonal antibodies (applied by hand) slides (up to 72 in a batch) are placed in racks and cycled through tanks of reagents, comprising ...
متن کاملTwo-colour immunoenzymatic technique using sequential staining by APAAP
Aims: To extend the alkaline phosphatase-antialkaline phosphatase (APAAP) immunoenzyme single stain method to a more generally applicable double stain technique. This will allow two primary antibodies of the same isotype ofIgG and specifically the nuclear antigen bromodeoxyuridine (BRdU) to be evaluated with a cell surface antigen identifier. Method: Sequential applications of the APAAP method ...
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introduction: the present study was undertaken to produce alkaline phosphatase anti- alkaline phosphatase (apaap) complex and to use it in one of the most practical methods of determining the site of antigen in tissue or in cell, i.e. apaap technique, and its comparison with similar foreign products. materials and methods: in this basic and applied study the secreted antibodies of the two hybri...
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Monoclonal antibodies have become increasingly important in the diagnosis and classification of neoplastic blood disorders. Immunofluorescent labelling of cell surface antigens has been the conventional method used for this purpose. However, this method of antigen detection presents a number of drawbacks when used in a routine hospital haematology laboratory: namely that blood or bone marrow sa...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1987
ISSN: 0021-9746
DOI: 10.1136/jcp.40.3.355