Simple methods of preparing nicotinamide mononucleotide

Enzymatic synthesis of polymers containing nicotinamide mononucleotide.

Nicotinamide mononucleoside 5'-diphosphate in its reduced form is an excellent substrate for polynucleotide phosphorylase from Micrococcus luteus both in de novo polymerization reactions and in primer extension reactions. The oxidized form of the diphosphate is a much less efficient substrate; it can be used to extend primers but does not oligomerize in the absence of a primer. The cyanide addu...

Nicotinamide mononucleotide pyrophosphorylase activity in animal tissues.

An enzyme which catalyzes the formation of nicotinamide mononucleotide in the presence of nicotinamide, S-phosphoribosylpyrophosphate, adenosine triphosphate, and Mgff has been purified from rat liver. The reaction is highly specific for ATP and is not substituted for by a variety of nucleotides. Enzymic activity has been found in all of the tissues studied, and in the crude homogenate it is ap...

Nicotinamide mononucleotide adenylyltransferase of pig-liver nuclei. The effects of nicotinamide mononucleotide concentration and pH on dinucleotide synthesis.

Identification of the nicotinamide mononucleotide adenylyltransferase of Trypanosoma cruzi

The intracellular parasite Trypanosoma cruzi is the aetiological agent of Chagas disease, a public health concern with an increasing incidence rate. This increase is due, among other reasons, to the parasite's drug resistance mechanisms, which require nicotinamide adenine dinucleotide (NAD+). Furthermore, this molecule is involved in metabolic and intracellular signalling processes necessary fo...

Functional Characterisation of Three Human Nicotinamide Mononucleotide Adenylyltransferases

Nicotinamide adenine dinucleotide, NAD, is an essential redox factor in many, primarily catabolic, hydrogen transfer reactions. While the redox reactions are not accompanied by a net loss of the pyridine nucleotides, NAD is actively degraded in a variety of ADP-ribosyl transfer reactions which are implicated, for examples, in cellular stress responses, calcium signalling and genomic stability. ...