SiFBA5, a cold-responsive factor from Saussurea involucrata promotes cold resilience and biomass increase in transgenic tomato plants under cold stress

Abstract Background Saussurea involucrata survives in extreme arctic conditions and is very cold-resistant. This species grows rocky, mountainous areas with elevations of 2400–4100 m, which are snow-covered year-round subject to freezing temperatures. S. ’s ability survive an low-temperature environment suggests that it has particularly high photosynthetic efficiency, providing a magnificent model, rich gene pool, for the analysis plant cold stress response. Fructose-1, 6-bisphosphate aldolase (FBA) key enzyme photosynthesis process also mediates conversion fructose 1, (FBP) into dihydroxyacetone phosphate (DHAP) glycerol triphosphate (GAP) during glycolysis gluconeogenesis. The molecular mechanisms underlying tolerance still unclear; therefore, our work aims investigate role FBA cold-stress Results In this study, we identified cold-responsive gene, SiFBA5 , based on preliminary low-temperature, genome-wide transcriptional profiling . Expression indicated temperatures rapidly induced expression suggesting participates initial Subcellular localization revealed localized chloroplast. Transgenic tomato plants overexpressed were generated using CaMV 35S promoter. Phenotypic observation suggested transgenic displayed increased efficiency comparison wild-type plants. Conclusion Cold detrimental impact crop yield. Our results demonstrated positively regulates response stress, great significance increasing yield under conditions.