Shotgun Mass Spectrometry Workflow Combining IEF and LC-MALDI-TOF/TOF
نویسندگان
چکیده
منابع مشابه
Shotgun Mass Spectrometry Workflow Combining IEF and LC-MALDI-TOF/TOF
We present a high throughput shotgun mass spectrometry workflow using a bidimensional peptide fractionation procedure consisting of isoelectric focusing and RP-HPLC prior to mass spectrometric analysis, with the aim of optimizing peptide separation and protein identification. As part of the workflow we used the 'Isotope-Coded Protein Labeling' (ICPL) method for accurate relative quantitation of...
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ژورنال
عنوان ژورنال: The Protein Journal
سال: 2010
ISSN: 1572-3887,1573-4943
DOI: 10.1007/s10930-009-9227-7