Short protocol for pulsed field gel electrophoresis of a variety ofClostridiaspecies
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چکیده
منابع مشابه
Short protocol for pulsed field gel electrophoresis of a variety of Clostridia species.
While pulsed field gel electrophoresis has become an important tool for genotyping of bacteria, one of its drawbacks is that standard methods are rather time-consuming. In order to overcome this problem, shortened procedures for DNA preparation have been developed for some bacterial species. The aim of this study was to examine if a short procedure used for pulsed field gel electrophoresis of C...
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DNA molecules in an agarose matrix elongate and migrate toward the anode when exposed to an electric field. An agarose matrix represents a highly irregular network with pores of various dimensions, large open areas, and regions with different densities. During electrophoresis, migration will be retarded by obstructions that prevent sieving by size and result in "end-on" migration as if through ...
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ژورنال
عنوان ژورنال: FEMS Immunology & Medical Microbiology
سال: 1999
ISSN: 0928-8244,1574-695X
DOI: 10.1111/j.1574-695x.1999.tb01296.x