Short antimicrobial peptidomimetic SAMP-5 effective against multidrug-resistant gram-negative bacteria

Abstract SAMP-5 is a short histidine-derived antimicrobial peptidomimetic with pendant dialkylated tail. In this study, we evaluated the potential of as an agent to combat multidrug-resistant gram-negative bacteria. showed potent activity (minimum inhibitory concentration 16-64 μg/ml) comparable melittin against Escherichia coli (MDREC) and (MDRPA). displayed no cytotoxicity three mammalian cells such mouse macrophage RAW264.7, embryonic fibroblast NIH-3T3, human bone marrow SH-SY5Y at 128 μg/ml. resistance proteolytic degradation pepsin, trypsin, α-chymotrypsin, proteinase K. Importantly, unlike ciprofloxacin, antibiotic arose for Pseudomonas aeruginosa during 7 days serial passage 0.5 × MIC. Moreover, synergy or additive effects MDRPA MDREC, when it combined chloramphenicol, oxacillin. Collectively, our results suggested that promising alternative adjuvant treat infections caused by