Shoot tip culture and thermotherapy for recovering virus-free plants of garlic
نویسندگان
چکیده
منابع مشابه
elimination of bean yellow mosaic virus through thermotherapy combined with meristem-tip culture in gladiolus corms
bean yellow mosaic virus (bymv, potyvirus, potyviridae) causes serious disease in gladiolus spp. in this work, the possibility of obtaining bymv free plant material from virus infected gladiolus corms was studied. thermotherapy, meristem-tip culture and combination of both techniques on infected corms/meristem-tip explants (0.5–1 mm in length) resulted in bymv elimination up to 15.38, 78.04 and...
متن کاملelimination of potyviruses from two commercial iranian garlic (allium sativum l.) populations through thermotherapy and by meristem tip culture
elimination of potyviruses from two commercial iranian garlic populations, hamedan and azar-shahr, through thermotherapy and by meristem tip culture were investigated. the infected bulbs of either one of the populations were initially treated at 30o c for one week and then at 36oc for 5 weeks. the meristems of cloves at two sizes of 0.4-0.6 mm and 0.6-0.8 mm were then cultured on ms medium supp...
متن کاملProduction of Indian Citrus Ringspot Virus Free Plants of Kinnow Employing Chemotherapy Coupled with Shoot Tip Grafting
Indian citrus ringspot virus (ICRSV) is known to cause serious problem in Kinnow (Citrus nobilis Lour × C. deliciosa Tenora). This paper reports the elimination of ICRSV from Kinnow by chemotherapy coupled with shoot tip grafting under in vitro conditions. Nodal segments from infected mother plant (indexed by indirect ELISA and RT-PCR) were cultured on MS medium containing 2-iP (1mg/l) and malt...
متن کاملUse of Tissue Culture Techniques for Producing Virus-Free Plant in Garlic and Their Identification through Real-Time PCR
This study was performed for comparison of meristem culture technique with shoot tip culture technique for obtaining virus-free plant, comparison of micropropagation success of two different nutrient media, and determination of effectiveness of real-time PCR assay for the detection of viruses. Two different garlic species (Allium sativum and Allium tuncelianum) and two different nutrient media ...
متن کاملImprovement of shoot-tip culture proliferation in banana using PEG6000. Leila Saeedavi, Ali Soleimani* and Mohammad Esmaeil Amiri
In order to improve the shoot proliferation rate of ‘Dwarf Cavendish’ banana shoot tip cultures, polyethylene glycol (PEG6000) was employed at concentrations of 2, 4, and 6% (w/v). The medium without PEG was considered as negative control. An increasing of shoot proliferation was achieved in MS medium containing 2% PEG. Furthermore, application of 2% PEG significantly increased the shoot dry we...
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ژورنال
عنوان ژورنال: Horticultura Brasileira
سال: 2000
ISSN: 0102-0536
DOI: 10.1590/s0102-05362000000300010