Shoot regeneration via culture of leaf explants in pear (Pyrus pyrifolia cv. Niitaka)

Pear Skin Stain Caused by Mycosphaerella graminicola on Niitaka Pear (Pyrus pyrifolia Nakai)

Pear skin stains on 'Niitaka' pears, which occur from the growing stage to the cold storage stage, reportedly negatively influence the marketing of pears. These stains on fruit skin are likely due to a pathogenic fungus that resides on the skin and is characterized by dark stains; however, the mycelium of this fungus does not penetrate into the sarcocarp and is only present on the cuticle layer...

Study of factors affecting direct shoot regeneration of pear (Pyrus communis L.)

Conventional methods of pear breeding, largely based on intra- and inter-specific hybridization, are difficult because pear is highly heterozygous, polygenic and has a long juvenile period. Genetic improvements of pear cultivars are possible through induction of mutations and gene transfer by genetic engineering. A general prerequisite for these approaches is to establish an efficient plant reg...

Study of Direct Adventitious Shoot Regeneration in Pomegranate ( Punicagranatum cv. MalasSaveh ) through Cotyledonary Explants

Punicagranatum cv. Malassaveh is one of the most desirable commercial cultivars in Iran. In thisstudy some experiments were conducted to introduce the best hormonal treatments for directadventitious shoot regeneration from mentioned cultivar via cotyledonary explants. For thispurpose, both liquid and agarMurashige and Skoog (1962) (MS) medium supplemented with BAat 2, 4.5, 9, 13 μM alone and in...

Effect of 5-Aminolevulinic Acid (ALA) on Leaf Diurnal Photosynthetic Characteristics and Antioxidant Activity in Pear (Pyrus Pyrifolia Nakai)

Agrobacterium-mediated genetic transformation using cotyledons in Japanese pear (Pyrus pyrifolia)

Genetic transformation was successfully established producing both transformed adventitious shoots and calli in Japanese pear (Pyrus pyrifolia Nakai) by using cotyledons as explants. Cotyledons of five cultivars were co-cultivated with Agrobacterium tumefaciens strain LBA4404 carrying the pBIN19-sgfp, which contained a green fluorescent protein gene and the neomycin phosphotransferase gene. In ...