Sesquiterpene lactones isolated from Carpesium abrotanoides L. by LC–MS combined with HSCCC inhibit liver cancer through suppression of the JAK2/STAT3 signaling pathway

Carpesium abrotanoides L. is rich in sesquiterpene lactones, the experiments of anti-tumor activity vitro showed that total lactone extracts exhibited most cytotoxicity activities against HepG-2 cells (IC50 = 4.2 μg/mL). In order to isolated active compounds quickly, strategy HPLC-ESI-Q-TOF-MS/MS combined with high-speed counter-current chromatography (HSCCC) was implemented. Three compounds, 2α,5α-dihydroxy-11αH-eudesma-4(15)-en-12,8β-olide (1), telekin (2), oxoeudesm-11(13)-eno-12,8α-lactone (3) were obtained. Their structures elucidated by detailed analysis 1D, 2D NMR and HRMS data. addition, 1–3 evaluated for their cytotoxic cell line IC50 value 9.83, 2.95, 4.15 μM, respectively. Moreover, all not only can reduce expression JAK2 STAT3 mRNA but also inhibit p-JAK2 p-STAT3 protein levels a dose‐dependent manner cells. conclusion, three inhibited proliferation via suppressing JAK2/STAT3 signaling pathway.