Serum PCR in diagnosis of neonatal sepsis caused by bacteria and enteroviruses.

Although blood culture is considered the gold standard for detection of neonatal infections bloodstream, its result can take from 48-72 h and has low sensitivity. In this study, we determined usefulness panbacterial polymerase chain reaction (PCR) panviral RT-PCR in serum, as tests early diagnosis sepsis. We studied 195 patients with clinical sepsis, up to age 28 days, hospitalized Neonatal Intensive Care Unit (NICU) Hospital Infantil de México “Federico Gómez”, umbilical cord samples obtained healthy newborns. Blood cultures, DNA / RNA extraction were done a system primers rRNA 16s region, which highly conserved large number bacteria non-coding enteroviral 5´ (5’-NCR) respectively. The results showed that among 90 newborns sepsis negative PCR, 19 randomly selected perform RT-PCR; 26% them had positive result. Statistical analysis included PCR test’s sensitivity, specificity, predictive value (PPV) (NPV) using standard. sensitivity was 77%, specificity 83%, PPV 92% NPV 59%. summary, useful, quick effective should be used complement microbiological diagnoses. frequency low, recommended. Our indicate molecular provides balance cost-benefit.