Serum-free cell culture: the serum-free media interactive online database
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Serum-free cell culture: the serum-free media interactive online database.
Fetal bovine serum (FBS) is a ubiquitously used essential supplement in cell culture media. However, there are serious scientific and ethical concerns about the use of FBS regarding its harvest and production. During the last three decades, FBS could be substituted by other supplements or by the use of defined chemical components in serum-free cell culture. A number of serum-free medium formula...
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Use of serum-free media and the related genera of animal-free and protein-free media has increased significantly over the past 15 years. This is particularly true in industrial applications, in which the use of serum presents a safety hazard and a source of unwanted contamination in the production of biopharmaceuticals (1). As the name suggests, serum-free media are prepared without animal seru...
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Ovomacroglobulin and LDL fraction of egg yolk were effective to maintain the growth of established human cancer cell lines (Kato III, QG-90, Raji). A serum-free medium (BEM-841) containing 10 mM HEPES, 50 micrograms/ml of ovomacroglobulin, 0.1% (V/V) of LDL fraction of egg yolk, 10(-8) M catalase, 5 micrograms/ml of insulin, 5 micrograms/ml of transferrin, 5 ng/ml of selenium, 1 mg/ml of BSA, a...
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Serum-free culture is now becoming the general trend of biological production. Its application in Vero cell culture is significant. In the present paper, we reviewed factors affecting Vero cell serum-free culture and several culture techniques. In light of these reviews, we outlined its extensive application prospect.
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any of these topics nor provides a broad, integrated view of the field of behavioral pharmacology. This text is 571 pages long, and the lack of real depth limits its utility to time-pressed readers. Publication of conference proceedings potentially promotes the rapid communication of research information. Yet this text appears in print almost a year after the conference. By this time, many of t...
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ژورنال
عنوان ژورنال: ALTEX
سال: 2010
ISSN: 1868-596X
DOI: 10.14573/altex.2010.1.53