Serological Identity of Potato Virus X (PVX) and PCR Characterization of Its Coat Protein (CP) Gene
نویسندگان
چکیده
منابع مشابه
Tobacco mosaic virus (TMV) and potato virus X (PVX) coat proteins confer heterologous interference to PVX and TMV infection, respectively.
Replication of Potato virus X (PVX) was reduced in transgenic protoplasts that accumulated wild-type coat protein (CPWT) of Tobacco mosaic virus (TMV) or a mutant CP, CP(T42W), that produced highly ordered states of aggregation, including pseudovirions. This reaction is referred to as heterologous CP-mediated resistance. However, protoplasts expressing a CP mutant that abolished aggregation and...
متن کاملHeterologous Expression of Potato Virus Y Coat Protein, Isolate Pot187
Background: The advent of recombinant DNA technology has facilitated heterologous expression of proteins from various sources in different host systems including Escherichia coli. If a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. Such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...
متن کاملPlant Translation Elongation Factor 1Bβ Facilitates Potato Virus X (PVX) Infection and Interacts with PVX Triple Gene Block Protein 1
The eukaryotic translation elongation factor 1 (eEF1) has two components: the G-protein eEF1A and the nucleotide exchange factor eEF1B. In plants, eEF1B is itself composed of a structural protein (eEF1Bγ) and two nucleotide exchange subunits (eEF1Bα and eEF1Bβ). To test the effects of elongation factors on virus infection, we isolated eEF1A and eEF1B genes from pepper (Capsicum annuum) and supp...
متن کاملCharacterization of Coat Protein Gene of Cucumber Mosaic Virus Isolates in Iran
Background: Cucumber mosaic virus (CMV) from the Bromoviridae family, is one of the most widespread plant viruses in the world. Objectives: In the present study tomato fields in Guilan, Isfahan, Khorasan Razavi, Khuzestan and Tehran provinces were surveyed to determine the presence of CMV subgroups during 2011-2012. Materials and Methods: Out of 305 symptomatic leaf samples ...
متن کاملPurification, Partial Characterization, and Serological Comparison of Soybean Mosaic Virus and its Coat Protein
SOONG, M. M., and G. M. MILBRATH. 1980. Purification, partial characterization, and serological comparison of soybean mosaic virus and its coat protein. Phytopathology 70:388-391. An Illinois isolate of soybean mosaic virus (SMV-IL) was purified from frequent length of 675-750 nm. Infectious SMV RNA was separated from cultivar Kanrich soybean leaves, 16-18 days after inoculation by protein bydi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: International Journal of Phytopathology
سال: 2013
ISSN: 2312-9344,2313-1241
DOI: 10.33687/phytopath.002.02.0264