منابع مشابه
Serial section scanning electron microscopy of adult brain tissue using focused ion beam milling.
Introduction Analyzing the synaptic basis of neuronal circuits within a volume of brain tissue requires electron microscopy. With a resolution capable of seeing the smallest syn-aptic contacts, this method uses different sectioning techniques to produce serial images suitable for seeing the ultrastruc-ture within significant volumes of brain tissue. Sections are cut from a block of tissue, leav...
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The morphology and the existence of a growth cycle of Mycoplasma pneumoniae have not been clearly established. There is disagreement as to whether this organism exists as a spherical or filamentous form, and whether it progresses from filamentous to spherical forms as the organism ages. A scanning-beam electron microscope (SEM) was utilized to provide detailed observations of the cycle of morph...
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A new method for the image acquisition in scanning electron microscopy (SEM) was introduced. The method used adaptively increased pixel-dwell times to improve the signal-to-noise ratio (SNR) in areas of high detail. In areas of low detail, the electron dose was reduced on a per pixel basis, and a-posteriori image processing techniques were applied to remove the resulting noise. The technique wa...
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Recently, three-dimensional reconstruction of ultrastructure of the brain has been realized with minimal effort by using scanning electron microscopy (SEM) combined with focused ion beam (FIB) milling (FIB-SEM). Application of immunohistochemical staining in electron microscopy (EM) provides a great advantage in that molecules of interest are specifically localized in ultrastructures. Thus, we ...
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Tomographic reconstruction of a wide range of materials and biological samples can be accomplished via serial-section broad-ion-beam tomography (SSBIB) – alternately ion-polishing away material and imaging the remaining polished surface with optical or scanning electron microscopy (SEM) [1]. Under computer control it is practical to acquire 2-D images of hundreds of sections without user interv...
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ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2018
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s1431927618007705