Separation of Polar Compounds using Poly(Ethylene Oxide) Bonded Stationary Capillary Liquid Chromatography

Poly(ethylene oxide) (PEO) bonded stationary phase has been synthesized by a single and simple step reaction. glycol monomethyl ether p-toluene sulfonate) (tosylated-PEO, molecular weight 900, n ≈ 18) was chemically to 3-aminopropyl silica (TSKgel NH2-60, 5 µm particle size, 60 Å mean pore diameter). The prepared able separate polar compounds such as phenolics nucleobases in capillary liquid chromatography. retention separation of could be achieved under isocratic elution condition. Nucleobases thymine, adenine, uracil, uridine, cytidine toluene (phenol, pyrocatechol, pyrogallol) were baseline separated less than 6 min using 98% acetonitrile 7 minutes 80% acetonitrile, respectively. We demonstrated that the analyte decreased with decreasing eluent concentration. these believed based on dipole-dipole and/or hydrogen-bonding interactions.