Separation of Marek's disease virus DNA by field inversion gel electrophoresis.
نویسندگان
چکیده
منابع مشابه
Separation of very large DNA molecules by gel electrophoresis.
Very large DNA molecules were separated by electrophoresis in horizontal slab gels of dilute agarose. Conditions of electrophoresis were developed using intact DNA molecules from the bacterial viruses lambda, T4 and G. Their DNAs have molecular weights (M) of 32 million, 120 million, and 500 million, respectively. Several electrophoresis conditions were found which give sufficiently high mobili...
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Standards commonly employed to calibrate &e excludon chromrrto@raphy cdunn, usually do not t h e ” have welldefined &os. Carefully characterized spherkal solutes In the apporprlate rlze rHI(J. are therefore of condderabk Interest. The chromatographk khavlor of carboxylated starburst dendrhers-characterlzed by quaslelaatk lbht scatterlng and vkomotry-on a Super080 &e exdudon cdunnhasbnnexpbred. ...
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Mobility of supercoiled (form I) and nicked circular (form II) plasmid DNAs was determined on two major forms of pulsed-field electrophoresis, CHEF and OFAGE. Plasmids with molecular lengths ranging from 2.30 to 17.8 kilobase pairs (kb) were used with Saccharomyces cerevisiae chromosomes as standards. Agarose gel concentrations were varied from 0.3 to 2.0 percent, with higher percentage gels re...
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A novel technique, which we call secondary pulsed field gel electrophoresis (SPFG) has been developed. In SPFG, short pulses are applied in the direction of net migration of the DNA in addition to the reorienting pulses used in conventional pulsed field electrophoresis (PFG). Experimental results show that SPFG extends and improves the electrophoretic resolution of DNA for molecules from 0.5 me...
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ژورنال
عنوان ژورنال: The Japanese Journal of Veterinary Science
سال: 1990
ISSN: 0021-5295,1881-1442
DOI: 10.1292/jvms1939.52.1301