Separation of human X- and Y-bearing sperm using free-flow electrophoresis.
نویسندگان
چکیده
منابع مشابه
Evaluation of Discontinues Puresperm Gradient in Separation of X And Y Bearing Human Spermatozoa by Fluorescent in Situ Hybridization (FISH)
Introduction: To evaluate the 8 layer discontinues puresperm gradient in separation of human spermatozoa according to sex chromosomes by fluorescent in situ hybridization (FISH). Material and Methods: This study was carried out on three patient referring to Royan fertility and infertility center. Semen analysis was assessed according to WHO criteria. Each sample was divided into two aliquots, ...
متن کاملSperm processing affected ratio of X- and Y-bearing sperm
Studies showed that assisted reproductive technology changed the sex ratio at birth, but the effect of semen processing (swim-up technique and density-gradient separation) on enriching either Xor Y-bearing sperm is always controversial. We recruited 30 healthy males and their semen samples were obtained by masturbation after sexual abstinence for 2-7 days. Each sample was divided into 3 aliquot...
متن کاملThe Effect of Diffrent Factors on X- and Y Bearing Sperm in Bull Semen
Background: Newborn gender ratio is one of the most important factors in livestock industry. This ratio has affected on economic condition and controls sex-linked genetically transferred diseases. Offspring sex ratio is affected by such various factors. One of these factors is the Y to X bearing sperm ratio in normal semen that is called primary sex ratio, season and individual differences are ...
متن کاملFlow cytometric determination of the proportions of X- and Y-chromosome-bearing sperm in samples of purportedly separated bull sperm.
A rapid assay for determining the proportions of X- and Y-chromosome-bearing sperm in semen samples would benefit research aimed at sex ratio control through sperm separation. It also would be of value for quality control should a separation technique be developed. Flow cytometric methods capable of measuring sperm DNA content precisely enough to resolve and quantify the X and Y populations in ...
متن کاملthe effects of follicular fluid on human x y bearing sperm ratio by fluorescent in situ hybridization (fish)
background: some evidences showed that the secretion of uterine tube vagina and follicular fluid (ff) affects x and y-chromosome populations. sperm selection with x or y chromosome can added to oocyte for gender desired. the isolation of x y sperm have done and all efforts in this field are done to make culture media similar to in vivo condition. the objective of this study was to find if the f...
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ژورنال
عنوان ژورنال: Proceedings of the Japan Academy, Series B
سال: 1983
ISSN: 0386-2208,1349-2896
DOI: 10.2183/pjab.59.276