Separation of Bile Acids with Reversed Phase Partition Chromatography.

The separation of the C12-C18 fatty acids by reversed-phase partition chromatography.

The resolution of mixtures of C16-C24 normal-chain fatty acids by reversed-phase partition chromatography.

Stand oils (fish drying oils) made from the body oil of the pilchard (Sardina ocelktta Jenyns) are of commercial importance in South Africa and, as part of a general study of these and other polymerized oils (cf. Joubert & Sutton, 1952), the need arose for an analytical method suitable for the separation of mixtures of saturated and unsaturated acids having from 16 to 24 carbon atoms in the cha...

Studies on bile acids: The microquantitative separation of cellular bile acids by gas-liquid chromatography.

1. A method is described for the quantitative isolation of bile acids from cellular material. Homogenates of rat liver are freeze-dried and extracted exhaustively with 95% (v/v) ethanol containing 0.1% (v/v) of aq. ammonia (sp.gr. 0.88) and purified by anion-exchange chromatography on Amberlyst A-26. 2. The extracted bile acid conjugates are subjected to either of two hydrolytic procedures, one...

Enantiomeric separation of Linezolid by chiral reversed-phase liquid chromatography.

A chiral liquid chromatographic method is developed for the enantiomeric resolution of Linezolid, (S)(-)-N-[[-3-(3-fluoro-4-(4-morpholinyl)phenyl]-2-oxo-5-oxazolidinyl] methyl] acetamide, an antibiotic in bulk drugs. The enantiomers of Linezolid are resolved on a Chiralcel OJ-RH column using a mobile phase system containing 150mM di-sodium hydrogen phosphate buffer (pH 4.5)-acetonitrile (86:14,...

Separation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column

The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...