Separation of Acid Mucopolysaccharides by Paper Electrophoresis.

The electrophoresis of acid mucopolysaccharides on filter paper.

Principles of Nucleic Acid Separation by Agarose Gel Electrophoresis

Agarose gel electrophoresis is a routinely used method for separating proteins, DNA or RNA. (Kryndushkin et al., 2003). Nucleic acid molecules are size separated by the aid of an electric field where negatively charged molecules migrate toward anode (positive) pole. The migration flow is determined solely by the molecular weight where small weight molecules migrate faster than larger ones (Samb...

Separation and determination of homovanillic acid and vanillylmandelic acid by capillary electrophoresis with electrochemical detection

A method of separation and determination of homovanillic acid (HVA) and vanillylmandelic acid (VMA) was developed based on capillary zone electrophoresis/amperometric detection with high sensitivity, good resolution and selectivity. In order to achieve complete separation and good response, several factors including pH, buffer concentration, separation voltage, detection potential and the lengt...

Determination of urinary 3-methoxy-4-hydroxymandelic acid (vanillylmandelic acid) by paper electrophoresis.

called vanillylmandelic acid (VMA), in urine has recently come into use as a confirmatory test for pheochromocytoma (1-6). Armstrong and his associates (1, 2, 7) discovered that this phenolic acid occurred in small amounts in normal human urine and in elevated concentrations in pheochromocytoma. As a result of their observations (1, 2) and especially of those by others in related investigations...

Cyclodextrine Screening for the Chiral Separation of Carvedilol by Capillary Electrophoresis

Carvedilol is administered as a racemic mixture of the R(+)- and S(-)-enantiomers, although it was demonstrated that the two enantiomers exhibit different pharmacological effects and stereoselective pharmacokinetics. The aim of this study was the evaluation of several native and derivatized cyclodextrines as chiral selectors for the separation of carvedilol enantiomers. Stereoselective interact...