Sensitive and high-throughput polyclonal antibody-based serological methods for rice stripe virus detection in both rice and small brown planthopper

Rice stripe virus (RSV) is a plant virus, and its infection leads to devastating disease that often causes serious yield losses in rice-producing regions. In this study, the capsid protein (CP) gene of RSV was cloned expressed E. coli (Rosetta), purified recombinant His-CP used immunize New Zealand white rabbits . After six weeks, polyclonal antibody against (PAb-CP ) obtained from serum immunized rabbits. Using prepared PAb-CP , three serology-based detection methods were developed, which could specifically test for RSV-infected rice plants or small brown planthoppers (SBPHs). Methods combine dot-blot with enzyme-linked immunosorbent assay high-throughput also developed detect seedlings SBPHs simultaneously wild fields. The field surveys indicated incidence suburbs Yangzhou City much lower than value ten years ago. This research presents multiple methods, using can identify conditions. Our study will benefit control planting • optimal titer coat 1 × 10 5 be by serological-based methods. both SBPs. Dot blot recommended