SEM Processing Of Blood Samples

How to study biological samples by FIB/SEM?

The focused ion beam (FIB)/scanning electron microscope (SEM) is a scanning microscope with an electron column and an ion column embedded in the same specimen chamber; both beams are aiming at the same point on the specimen surface. The FIB, generated by a Ga Liquid Metal Ion Source (LMIS), impacts the sample normal to the surface and can be focused to a spot as small as few nanometres. The FIB...

SEM Study on Old-styled Thai Gold Mosaic Glass Samples

Gold Mosaic glass has been used to decorate items and places since ancient time. The glass structure is divided into three layers: cover glass, gold layer and glass substrate. In this study, the composition of old-styled Thai gold mosaic glass samples was analyzed using SEM/EDX and Raman spectroscopy. It was found that the glass composition was sodiumsilicate based glass. Raman spectroscopy als...

Pre-PCR processing of samples.

Effect of delayed processing of blood samples on performance of cytomegalovirus antigenemia assay.

This prospective, parallel, and blind study of 120 blood samples from immunocompromised patients examined the influence of delayed sample processing on cytomegalovirus antigenemia assay. Cytomegalovirus was detected in 49 samples (40.8%): 41 were processed within 2 to 4 h, and 40 were reprocessed the following day. Results were discrepant in 17 of the samples with the lowest positive cell count...

Automated processing of whole blood samples into microliter aliquots of plasma

A rotor that accepts and automatically processes a bulk aliquot of a single blood sample into multiple aliquots of plasma has been designed and built. The rotor consists of a central processing unit, which includes a disk containing eight precision-bore capillaries. By varying the internal diameters of the capillaries, aliquot volumes ranging 1 to 10 mul can be prepared. In practice, an unmeasu...