Self-assembling of chimeric mussel-inspired bio-adhesives originated from Mytilus californianus and Anabaena flos-aquae: A new approach to develop underwater adhesion

Bio-adhesives play a pivotal role in wide range of medical applications. However, there are some problems about their application different pH values and low adhesion force under wet conditions. Here, we report new recombinant fusion protein achieved by mussel foot proteins (Mfps) Mytilus Californianus gas vesicle A (GvpA) Anabaena flos-aquae genetic engineering methods. Transmission electron microscopy verified that these chimeric self-assembled into β-sheet rich fibres because GvpA amyloid structure. Also, comparison to other studies, forces were significantly increased, especially the alkaline environments based on Mfp-3 Mfp-5, confirmed colloidal probe atomic microscope. This study illustrates copolymer Mfp-5-GvpA:GvpA-Mfp-3 can be used as sturdy underwater adhesive with tolerance auto-oxidation, at basic