Selective stimulation by mouse spleen cell conditioned medium of human eosinophil colony formation
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چکیده
منابع مشابه
Selective stimulation by mouse spleen cell conditioned medium of human eosinophil colony formation.
Stimulation of unfractionated or nonadherent human marrow cells in agar culture by pokeweed-mitogen-stimulated BALB/c mouse spleen cell conditioned medium (SCM) led, in most cultures, to the exclusive formation of eosinophil colonies. The culture system exhibited linearity of eosinophil colony formation with varying numbers of cells cultured, and the absolute numbers and size of SCM-stimulated ...
متن کاملStimulation by human placental conditioned medium of hemopoietic colony formation by human marrow cells.
Medium conditioned by human placental tissue was found to stimulate granulocytic and monocytic colony formation by human marrow cells in semisolid agar cultures. The colony-stimulating activity of unfractionated conditioned medium was equivalent to the activity of standard peripheral blood underlayers. Placentas were a reliable source of active material, and one placenta provided enough materia...
متن کاملPurification of a multipotential colony-stimulating factor from pokeweed mitogen-stimulated mouse spleen cell conditioned medium.
A factor able to stimulate the proliferation and differentiation of multipotential stem cells and progenitor cells of the granulocyte-macrophage, eosinophil, and erythroid lineages as well as being able to maintain factor-dependent cell lines in culture has been purified from pokeweed mitogen-stimulated mouse spleen cell-conditioned medium. The factor was purified over 2 million-fold by sequent...
متن کاملStimulation of eosinophil production in vitro by eosinophilopoietin and spleen-cell-derived eosinophil growth-stimulating factor.
Eosinophilopoietin (EPP) was previously characterized by the ability to stimulate eosinophil production in vivo, but these studies could not ascertain whether EPP had a direct effect on the bone marrow or acted indirectly by causing release of eosinophilopoietic activity by other tissues. The present studies demonstrate that EPP stimulates eosinophil growth in liquid culture of mouse bone marro...
متن کاملGranulocyte/macrophage-, megakaryocyte-, eosinophil- and erythroid-colony-stimulating factors produced by mouse spleen cells.
The formation of mature haemopoietic cells is controlled by hormones that specifically stimulate the progenitor cells of the granulocyte/macrophage, eosinophil, megakaryocyte and erythroid pathways. PWMSC medium (pokeweed-mitogen-stimulated spleen-cell-conditioned medium) is known to contain the biological activities that control the clonal proliferation of these four progenitor cells in vitro ...
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ژورنال
عنوان ژورنال: Blood
سال: 1983
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v61.5.999.999