Selective extraction of gonococcal envelope antigens with thiocyanates

Heat-labile antigens of Salmonella enteritidis. I. Extraction of antigens.

Milne, Margaret (University of Adelaide, Adelaide, Australia), and F. M. Collins. Heat-labile antigens of Salmonella enteritidis. I. Extraction of antigens. J. Bacteriol. 92:543-548. 1966.-Salmonella enteritidis strains of high and low mouse virulence were grown in continuous culture. Cell walls were obtained from both strains by sonic disruption, and the washed walls were extracted at 4 C with...

Extraction of tissue antigens for functional assays.

Many of the antigen targets of adaptive immune response, recognized by B and T cells, have not been defined (1). This is particularly true in autoimmune diseases and cancer(2). Our aim is to investigate the antigens recognized by human T cells in the autoimmune disease type 1 diabetes (1,3,4,5). To analyze human T-cell responses against tissue where the antigens recognized by T cells are not id...

The Melting Mechanism of the Alkali Thiocyanates

where Ws and WL represent the number of independent ways of realizing the arrangements of solid and melt respectively and Sf is the entropy of fusion. Each new kind of disorder which occurs on passing from the crystal to the melt involves a new mechanism of randomization on melting. Ubbelohde! has summarized the different ways of randomization in the transition from the solid to the melt as pos...

Selective extraction spectrophotometric determination

Neutralizing and protective antibodies directed against vaccinia virus envelope antigens.

The infection mechanism of vaccinia virus is largely unknown. Neither the attachment protein of extracellular enveloped virus (EEV), the biologically relevant infectious form of the virus, nor its cellular receptor has been identified. Surprisingly, all former attempts using antibodies to block EEV infection of cells in vitro had failed. Here, we report the production of an anti-envelope hyperi...