Selective dye and ionic permeability of gap junction channels formed by connexin45.
نویسندگان
چکیده
منابع مشابه
Selective Dye and Ionic Permeability of Gap Junction Channels Formed by Connexin 45 Richard
Gap junctions are thought to mediate the direct intercellular coupling of adjacent cells by the gating of an aqueous pore permeable to ions and molecules of up to 1 kD or 8 to 14 A in diameter. We performed ion-substitution and dye-transfer experiments to determine the relative Cl-/K conductance and dye permeability of anionic fluorescein derivatives in chick connexin45 (Cx45) channels. We demo...
متن کاملSelective permeability of gap junction channels.
Gap junctions mediate the transfer of small cytoplasmic molecules between adjacent cells. A family of gap junction proteins exist that form channels with unique properties, and differ in their ability to mediate the transfer of specific molecules. Mutations in a number of individual gap junction proteins, called connexins, cause specific human diseases. Therefore, it is important to understand ...
متن کاملConnexin43 and connexin45 form heteromeric gap junction channels in which individual components determine permeability and regulation.
Two gap junction proteins, connexin43 (Cx43) and connexin45 (Cx45), are coexpressed in many cardiac and other cells. Homomeric channels formed by these proteins differ in unitary conductance, permeability, and regulation. We sought to determine the ability of Cx43 and Cx45 to oligomerize with each other to form heteromeric gap junction channels and to determine the functional and regulatory pro...
متن کاملGap junction channels formed by coexpressed connexin40 and connexin43.
Many cardiovascular cells coexpress multiple connexins (Cx), leading to the potential formation of mixed (heteromeric) gap junction hemichannels whose biophysical properties may differ from homomeric channels containing only one connexin type. We examined the potential interaction of connexin Cx43 and Cx40 in HeLa cells sequentially stably transfected with these two connexins. Immunoblots verif...
متن کاملElectrical conductance of mouse connexin45 gap junction channels is modulated by phosphorylation.
In this study we report about the modulation of connexin45 (Cx45) gap junction channel properties by phosphorylation of the connexin molecules through different protein kinases. Phosphorylation of Cx45 was studied in HeLa cells transfected with mouse Cx45 (mCx45). Using Western blotting (WB) and immunocytochemistry, these cells were found exclusively positive for Cx45 and the protein was separa...
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ژورنال
عنوان ژورنال: Circulation Research
سال: 1994
ISSN: 0009-7330,1524-4571
DOI: 10.1161/01.res.75.3.483