Selective Cleavage of BLM, the Bloom Syndrome Protein, during Apoptotic Cell Death

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Transcriptomic and Protein Expression Analysis Reveals Clinicopathological Significance of Bloom Syndrome Helicase (BLM) in Breast Cancer.

Bloom syndrome helicase (BLM) has key roles in homologous recombination repair, telomere maintenance, and DNA replication. Germ-line mutations in the BLM gene causes Bloom syndrome, a rare disorder characterized by premature aging and predisposition to multiple cancers, including breast cancer. The clinicopathologic significance of BLM in sporadic breast cancers is unknown. We investigated BLM ...

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DNA degradation during apoptotic cell death

Apoptosis is a principal mechanism in metazoans by which superfluous or potentially harmful cells are eliminated. Deregulation of this process leads to a variety of diseases such as cancer and autoimmune diseases. Stimuli that can induce apoptosis are relatively diverse, and include the death factors (Fas ligand, tumor necrosis factor and TRAIL), DNA damage, and oxidative stress. Regardless of ...

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The extracellular release of HMGB1 during apoptotic cell death.

High mobility group box 1 protein (HMGB1) is a non-histone nuclear protein with dual function. Inside the cell, HMGB1 binds DNA and regulates transcription, whereas outside the cell, it serves as a cytokine and mediates the late effects of LPS. The movement of HMGB1 into the extracellular space has been demonstrated for macrophages stimulated with LPS as well as cells undergoing necrosis but no...

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Garlic Extract Can Induce Apoptotic Cell Death in The Human Colon Adenocarcinoma HT29 Cell Line

  Background and Objective: Garlic has been known worldwide as a dietary constituent with many pharmacological effects. The present in vitro study was designed to investigate the putative anticancer effect of garlic extract on cancer cells, and if this effect was through apoptosis induction. Material and Methods:  Human colon adenocarcinoma cells HT29 were treated with different doses (1, 5 a...

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Cellular defects caused by hypomorphic variants of the Bloom syndrome helicase gene BLM.

BACKGROUND Bloom syndrome is an autosomal recessive disorder characterized by extraordinary cancer incidence early in life and an average life expectancy of ~27 years. Premature stop codons in BLM, which encodes a DNA helicase that functions in DNA double-strand-break repair, make up the vast majority of Bloom syndrome mutations, with only 13 single amino acid changes identified in the syndrome...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 2001

ISSN: 0021-9258

DOI: 10.1074/jbc.m006462200