Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR
نویسندگان
چکیده
منابع مشابه
Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR
Background: Considering the broad variation in the expression of housekeeping genes among tissues and experimental situations, studies using quantitative RT-PCR require strict definition of adequate endogenous controls. For glioblastoma, the most common type of tumor in the central nervous system, there was no previous report regarding this issue. Results: Here we show that amongst seven freque...
متن کاملSelection of housekeeping genes as internal controls for quantitative RT-PCR analysis of the veined rapa whelk (Rapana venosa)
BACKGROUND The veined rapa whelk Rapana venosa is an important commercial shellfish in China and quantitative real-time PCR (qRT-PCR) has become the standard method to study gene expression in R. venosa. For accurate and reliable gene expression results, qRT-PCR assays require housekeeping genes as internal controls, which display highly uniform expression in different tissues or stages of deve...
متن کاملValidation of putative housekeeping genes for gene expression studies in human hepatocellular carcinoma using real-time quantitative RT-PCR
Validation of putative housekeeping genes for gene expression studies in human hepatocellular carcinoma using real-time quantitative RT-PCR. BMC Cancer 2008, 8:350 doi:10.1186/1471-2407-8-350 Vito R Cicinnati ([email protected]) Qingli Shen ([email protected]) Georgios C Sotiropoulos ([email protected]) Arnold Radtke ([email protected]) Guido Gerken ([email protected]...
متن کاملInvestigation of Reference Genes in Vibrio parahaemolyticus for Gene Expression Analysis Using Quantitative RT-PCR
Vibrio parahaemolyticus is a significant human pathogen capable of causing foodborne gastroenteritis associated with the consumption of contaminated raw or undercooked seafood. Quantitative RT-PCR (qRT-PCR) is a useful tool for studying gene expression in V. parahaemolyticus to characterize its virulence factors and understand the effect of environmental conditions on its pathogenicity. However...
متن کاملEvaluation of Reference Genes for Gene Expression Analysis Using Quantitative RT-PCR in Azospirillum brasilense
Azospirillum brasilense is a nitrogen fixing bacterium that has been shown to have various beneficial effects on plant growth and yield. Under normal conditions A. brasilense exists in a motile flagellated form, which, under starvation or stress conditions, can undergo differentiation into an encapsulated, cyst-like form. Quantitative RT-PCR can be used to analyse changes in gene expression dur...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Molecular Biology
سال: 2009
ISSN: 1471-2199
DOI: 10.1186/1471-2199-10-17