Selection of optimal DNA oligos for gene expression arrays
نویسندگان
چکیده
منابع مشابه
Selection of optimal DNA oligos for gene expression arrays
MOTIVATION High density DNA oligo microarrays are widely used in biomedical research. Selection of optimal DNA oligos that are deposited on the microarrays is critical. Based on sequence information and hybridization free energy, we developed a new algorithm to select optimal short (20-25 bases) or long (50 or 70 bases) oligos from genes or open reading frames (ORFs) and predict their hybridiza...
متن کاملDNA arrays for analysis of gene expression.
Introduction DNA microarrays, microscopic arrays of large sets of DNA sequences immobilized on solid substrates, are valuable tools in areas of research that require the identification or quantitation of many specific DNA sequences in complex nucleic acid samples. DNA microarrays, which come in an ever increasing variety of flavors, have been used in genetic mapping studies, mutational analyses...
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DNA hybridization arrays [also known as macroarrays, microarrays and/or high-density oligonucleotide arrays (Gene Chips)] bring gene expression analysis to a genomic scale by permitting investigators to simultaneously examine changes in the expression of literally thousands of genes. For hybridization arrays, the general approach is to immobilize gene-specific sequences (probes) on a solid stat...
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High-density DNA oligo microarrays are widely used in biomedical research. In this paper we describe algorithms to optimize the selection of specific probes for each gene in an entire genome. Having optimized probes for each gene is valuable for two reasons. By minimizing background hybridization they provide more accurate determinations of true expression levels. And having optimum probes elim...
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ژورنال
عنوان ژورنال: Bioinformatics
سال: 2001
ISSN: 1367-4803,1460-2059
DOI: 10.1093/bioinformatics/17.11.1067