Selection of functional EPHB2 genotypes from ENU mutated grass carp treated with GCRV

Abstract Background N -ethyl- -nitrosourea (ENU) mutagenesis is a useful method for the genetic engineering of plants, and production functional mutants in animal models including mice zebrafish. Grass carp reovirus (GCRV) haemorrhagic disease grass which has caused noteworthy losses fingerlings over last few years. To overcome this problem, we used ENU mutant an attempt to identify resistance genes future hereditary rearing projects carp. Results This study ENU-mutated markers associated with by GCRV. Bulked segregant analysis (BSA) was performed on two homozygous gynogenetic groups who were susceptible or resistant identified 466,162 SNPs 197,644 InDels within genomes these mixed pools total 170 annotated region, 49 non-synonymous mutations at SNP sites 25 frame shift InDel sites. Of mutated genes, 5 randomly selected immune-related shown be more strongly expressed group as compared animals. In addition, found that one gene, EPHB2 , presented heterozygous altered animal’s responded GCRV disease. These intron region positions 5859 (5859 G > A ) 5968 (5968 significantly ( p = 0.002, 0.003) also correlate GCRV-resistant phenotype We evaluated mortality different fish genotypes response . The outcomes evaluations will selections breeding Conclusion Our results provide proof concept application BSA-sequence detecting responsible specific may help develop better methods marker-assisted selection, especially