Selection and validation of reference genes for RT-qPCR analysis in the pericarp of Litchi chinensis

Real-time reverse transcription quantitative PCR (RT-qPCR) is an important tool for gene expression analysis. Suitable reference genes are the basis of accurate and reliable RT-qPCR results. Litchi (Litchi chinensis Sonn.) a commercially tropical subtropical fruit, but rapid pericarp browning substantial negative impact on its commercial use. Reference validation could help in screening involved mechanism. We assessed 15 new candidate from litchi transcriptome to determine stable analysis pericarps different cultivars, with differing postharvest storage, under pathogenic stress. Ct values, geNorm, Normfinder, RefFinder algorithms, were used identify most transcription. GAGA-25 was comparing varieties fresh pericarp. HDAC9 STAM inoculated Of genes, across complete sample set. This study evaluated stability work provides foundation using qPCR function molecular mechanism studies browning.