Selection and use of reference genes in mouse mammary glands
نویسندگان
چکیده
منابع مشابه
Selection and use of reference genes in mouse mammary glands.
Obtaining quantitative data concerning gene expression is important for understanding milk synthesis in mammary glands. Quantitative real-time PCR (qRT-PCR) is an efficient tool to calculate gene expression; however, it is necessary to find valid reference genes for normalization of qRT-PCR data. We applied the geNorm software to eight commonly used reference genes to identify the most stable a...
متن کاملDifferential Expression of Alpha S1 Casein and Beta-Lactoglobulin Genes at Different Physiological stages of the Adani Goats Mammary Glands
Background: Milk proteins genes have been the focus of the researches as the candidate target genes that play a decisive role when animal breeding is desired.Objectives: In the present study, the transcriptional levels of Beta-lactoglobulin (BLG) and Alpha S1 casein (CSN1S1) genes were investigated during prenatal, milking and drying times in mammary glands of the Adani goats which showed...
متن کاملSelection of reference genes in mouse embryos and in differentiating human and mouse ES cells.
Embryonic Stem (ES) cells have the potential to form every cell of the body and thus are of great promise for tissue transplantation. One of the rising techniques that allows studying the differentiation state of ES cells is quantitative RT-PCR (qRT-PCR). When relative quantification by qRT-PCR is applied, accurate normalization is necessary, since differentiated embryonic stem cells and develo...
متن کاملUltrasonographic Examination of Mammary Glands in Caspian Mares during the Lactation and Dry Period
Objective- The aim of this study was to determine the normal ultrasonographic and morphologic appearance of mammary gland and teat in Caspian mares during lactation and dry period. Design- Experimental study. Animals- Seven mature Caspian mares Procedures- Using a 6.5-8.5 MHz linear transducer the ultrasonographic appearance of lactating and dry mammary glands was determined in 7 mature Casp...
متن کاملphylogeography and genetic diversity of the lesser mouse- eared bat (myotis blythii) in iran
in current study, 63 samples of bat populations collected from differ regions were used for evaluating the geographic variations. twenty cranial and dental characters for traditional morphometric and landmarks method on the ventral, dorsal skull and mandible for geometry morphometric studies were used. statistical analyses of traditional morphometric and geometry morphometric data indicated low...
ذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Genetics and Molecular Research
سال: 2010
ISSN: 1676-5680
DOI: 10.4238/vol9-1gmr724