Selection and characterization of the novel anti-human PD-1 FV78 antibody from a targeted epitope mammalian cell-displayed antibody library

Epitope Screening by Use of a Random Peptide-Displayed Phage Library and Polyclonal Antibody-Coupled Liposomes

Yoichi KUMADA, Masao NOGAMI, Fida HASAN, Masaaki TERASHIMA and Shigeo KATOH Department of Bioscience and Biotechnology, Okayama University, 1-1, Tsushimanaka 3, Okayam-shi, Okayama 700-8530, Japan Graduate School of Science and Technology, Kobe University, 1-1, Rokkodaicho, Kobe-shi, Hyogo 657-8502, Japan Graduate school of Human Sciences, Kobe College, 4-1, Okadayama, Nishinomiya-shi, Hyogo 66...

Development of a Novel Inhibitory Chimeric Anti-HER2 Monoclonal Antibody

Anti-epitope antibody, a novel site-directed antibody against human acetylcholinesterase.

AIM To construct synthetic antigens using the epitope of human brain acetylcholinesterase (hbAChE) for induction and detection of the specific antibody against the epitope, and to analyse the immunogenicity of the antibody. METHODS The epitope (RTVLVSMNYR, amino acids 143-152) of hbAChE was chemically synthesized, coupled with the carrier protein keyhole limpet hemocyanin (KLH) to construct a...

Characterization of the Anti-PD-1 Antibody REGN2810 and Its Antitumor Activity in Human PD-1 Knock-In Mice.

The Programmed Death-1 (PD-1) receptor delivers inhibitory checkpoint signals to activated T cells upon binding to its ligands PD-L1 and PD-L2 expressed on antigen-presenting cells and cancer cells, resulting in suppression of T-cell effector function and tumor immune evasion. Clinical antibodies blocking the interaction between PD-1 and PD-L1 restore the cytotoxic function of tumor antigen-spe...

Broad epitope coverage of a human in vitro antibody library

Successful discovery of therapeutic antibodies hinges on the identification of appropriate affinity binders targeting a diversity of molecular epitopes presented by the antigen. Antibody campaigns that yield such broad "epitope coverage" increase the likelihood of identifying candidates with the desired biological functions. Accordingly, epitope binning assays are employed in the early discover...