منابع مشابه
Drosophila Polyribosomes
Two populations of polyribosomes have been isolated from third instar larvae of D. melanogaster. One population appeared to be soluble while the second seemed membrane-bound. Short-term labeling of the two RNP fractions with radioactive nucleic acid and protein precursors was achieved by using a feeding stimulant. RNA was extracted from both polyribosomal fractions following 25, 40, and 60 min ...
متن کاملPolyribosomes of Escherichia coli
Growing cells incubated in a medium lacking glucose undergo a rapid breakdown of polyribosomes to single ‘70 S ribosomes. This conversion is accompanied by a loss of protein-synthetic capacity in vivo and is inhibited by chloramphenicol, but not by puromycin. The conversion of polysomes to 70 S ribosomes, with a half-life of 1: to 24 min at 37’, appears to reflect a breakdown of messenger ribon...
متن کاملPolyribosomes of Escherichia coli
Growing cells incubated in a medium lacking glucose undergo a rapid breakdown of polyribosomes to single ‘70 S ribosomes. This conversion is accompanied by a loss of protein-synthetic capacity in vivo and is inhibited by chloramphenicol, but not by puromycin. The conversion of polysomes to 70 S ribosomes, with a half-life of 1: to 24 min at 37’, appears to reflect a breakdown of messenger ribon...
متن کاملPolyribosomes from Tetrahymena
The free-living, ciliated protist Tetrahymena, which can be grown in synchronized cultures, lends itself to studies of protein synthesis and organelle morphogenesis in eukaryotic cells . The isolation of polyribosomes, the sites of protein synthesis, from Tetrahymena was reported by Whitson et al . (5) . After lysis of the cells by indole-saturated buffer, the polysomes were isolated in 0 .01 M...
متن کاملAnalytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium.
Analytical ultracentrifugation (AUC) is a versatile and powerful method for the quantitative analysis of macromolecules in solution. AUC has broad applications for the study of biomacromolecules in a wide range of solvents and over a wide range of solute concentrations. Three optical systems are available for the analytical ultracentrifuge (absorbance, interference, and fluorescence) that permi...
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ژورنال
عنوان ژورنال: The Journal of Kansai Medical University
سال: 1969
ISSN: 0022-8400
DOI: 10.5361/jkmu1956.21.4_138