Second-harmonic-generation imaging of tissue collagen and application to skin measurement
نویسندگان
چکیده
منابع مشابه
Second-harmonic imaging of collagen.
Molecules that have no center of symmetry are able to convert light to its second harmonic, at twice the frequency and half the wavelength. This only happens with any efficiency at very high light intensities such as are given by a pulsed laser, and because the efficiency of the process depends on the square of the intensity, it will be focal plane selective in exactly the same way as two-photo...
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We performed Second Harmonic Generation (SHG) imaging microscopy of endogeneous myosin-rich and collagen-rich tissues in amphibian and mammals. We determined the relative components of the macroscopic susceptibility tensor chi((2)) from polarization dependence of SHG intensity. The effective orientation angle theta(e) of the harmonophores has been determined for each protein. For myosin we foun...
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ژورنال
عنوان ژورنال: The Review of Laser Engineering
سال: 2008
ISSN: 0387-0200,1349-6603
DOI: 10.2184/lsj.36.214