Screening for protein–protein interactions with asymmetrical flow field-flow fractionation
نویسندگان
چکیده
منابع مشابه
Miniaturization of frit inlet asymmetrical flow field-flow fractionation.
A miniaturized frit inlet asymmetrical flow field-flow fractionation (mFI-AFlFFF) channel has been constructed and tested for the separation of proteins. By scaling down the geometrical channel dimension of a conventional FI-AFlFFF system, flow rate ranges that can be manipulated were decreased to 20-30 microL/min, which reduces the injection amount of sample materials. The end effect contribut...
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Asymmetrical Flow Field-Flow Fractionation (AF4) is a separation technique applicable to particles over a wide size range. Despite the many advantages of AF4, its adoption in routine particle analysis is somewhat limited by the large footprint of currently available separation cartridges, extended analysis times and significant solvent consumption. To address these issues, we describe the fabri...
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ژورنال
عنوان ژورنال: Journal of Pharmaceutical Sciences
سال: 2021
ISSN: 0022-3549
DOI: 10.1016/j.xphs.2021.02.026