Screening cell surface receptors using micromosaic immunoassays

Competitive immunoassays for simultaneous detection of metabolites and proteins using micromosaic patterning.

New high-throughput immunoassay methods for rapid point-of-care diagnostic applications represent an unmet need and current focus of numerous innovative methods. We report a new micromosaic competitive immunoassay developed for the analysis of the thyroid hormone thyroxine (T4), inflammation biomarker C-reactive protein (CRP), and the oxidative damage marker 3-nitrotyrosine (BSA-3NT) on a silic...

Magnetic-Nanoparticle-Based Immunoassays-on-Chip: Materials Synthesis, Surface Functionalization, and Cancer Cell Screening

and dormant cancer cell, [ 12 ] are often rare in a mixture of heterogeneous cells (usually less than 1% of the total cell number). The low number of target cells in blood sample largely limits the detection capability and reduces effectiveness of the time-sensitive cancer treatment. Therefore, the isolation and detection of the rare cells for early diagnosis and prognosis of cancers have drawn...

Screening for interference in immunoassays.

BACKGROUND The presence of interfering substances in patient samples submitted for immunoassay cannot be reliably anticipated. We therefore evaluated three interference screening techniques and estimated the prevalence of interfering substances as defined by positive outcomes with these protocols. METHODS We evaluated 160 samples for the presence of substances that may interfere with four imm...

Screening for interference in immunoassays.

P-184: The Role of Cell Surface Toll Like Receptors in Endometriosis

Background: Toll like receptors (TLRs) are a major family of innate immune systems which recognize specific pathogen associated molecular patterns (PAMPS)in bacterial, fungi, virus and parasites. Human TLRs comprise a large family of 10 different type proteins that are expressed on various immune cells. Among these receptors, TLR1, 2, 4, 5, 6 and 10 were expressed on the cell surface. TLR2 form...